|
Free-flow electrophoresis (FFE), also known as carrier-free electrophoresis, is a continuous electrophoretic and liquid-based separation technique. It is typically used for quantitative and semiquantitative separations of proteins, peptides, organelles, cells, DNA origami and particles. The advantage of FFE is that the separation is conducted in a fast and gentle liquid-based manner, without any interaction of a solid matrix, like polyacrylamide in gel electrophoresis. This ensures a very high recovery rate because no analytes can get lost. FFE separations are continuous, which ensures a high throughput of analytes for preparative applications. Furthermore, the separations can be conducted under native or denaturing conditions.〔P.D. Patel and G. Weber, Electrophoresis in free fluid: a review of the technology and agrifood applications, J. Biol. Phys. Chem. 2003, 3, 60–73.〕 == Technique == An even and laminar liquid film is conducted between two plates, split up in parallel fractionation tubes, and collected in microtiter plates. A high voltage electric field is applied perpendicular to the laminar flow. Analytes in the laminar flow are separated by charge density and isoelectric point. Three different electrophoretic techniques can be applied: * Isoelectric focusing, or separation according to the isoelectric point. * Zone electrophoresis, or separation according to the net charge density. * Isotachophoresis, or separation according to the electrophoretic mobility. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Free-flow electrophoresis」の詳細全文を読む スポンサード リンク
|